1JKG
Structural basis for the recognition of a nucleoporin FG-repeat by the NTF2-like domain of TAP-p15 mRNA nuclear export factor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-12-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 104.340, 104.340, 170.344 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.900 |
| R-factor | 0.219 * |
| Rwork | 0.219 |
| R-free | 0.22500 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 * |
| RMSD bond angle | 1.235 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.071 * | 0.421 |
| Total number of observations | 301962 * | |
| Number of reflections | 43776 | |
| <I/σ(I)> | 9.4 | |
| Completeness [%] | 99.9 | 93.3 * |
| Redundancy | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8.5 * | 18 * | Na/K tartrate, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
| 1 | Vapor diffusion * | 8.5 * | 18 * | Na/K tartrate, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 12 (mg/ml) | |
| 2 | 1 | drop | Tris | 50 (mM) | |
| 3 | 1 | drop | 50 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 0.5 (mM) | |
| 5 | 1 | reservoir | sodium potassium tartrate | 0.8 (M) | |
| 6 | 1 | reservoir | MES | 100 (mM) |
