1JKG

Structural basis for the recognition of a nucleoporin FG-repeat by the NTF2-like domain of TAP-p15 mRNA nuclear export factor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2000-12-01
Detector	MARRESEARCH
Wavelength(s)	0.934
Spacegroup name	P 65 2 2
Unit cell lengths	104.340, 104.340, 170.344
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 1.900
R-factor	0.219 *
Rwork	0.219
R-free	0.22500
Structure solution method	MAD
RMSD bond length	0.005 *
RMSD bond angle	1.235 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	30.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.071 *	0.421
Total number of observations	301962 *
Number of reflections	43776
<I/σ(I)>	9.4
Completeness [%]	99.9	93.3 *
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	8.5 *	18 *	Na/K tartrate, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 291K
1	Vapor diffusion *	8.5 *	18 *	Na/K tartrate, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 291K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12 (mg/ml)
2	1	drop	Tris	50 (mM)
3	1	drop		50 (mM)
4	1	drop	dithiothreitol	0.5 (mM)
5	1	reservoir	sodium potassium tartrate	0.8 (M)
6	1	reservoir	MES	100 (mM)