1JKF
Holo 1L-myo-inositol-1-phosphate Synthase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 123 |
| Detector technology | CCD |
| Collection date | 2000-03-28 |
| Wavelength(s) | 0.9794, 0.9796, 0.9464 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 151.042, 95.959, 121.291 |
| Unit cell angles | 90.00, 126.04, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.400 |
| R-factor | 0.205 * |
| Rwork | 0.205 |
| R-free | 0.24300 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 * |
| RMSD bond angle | 1.927 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 500.000 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.048 * | 0.338 * |
| Total number of observations | 91546 * | |
| Number of reflections | 49388 | |
| <I/σ(I)> | 12.6 | 2 |
| Completeness [%] | 94.5 | 92.6 |
| Redundancy | 1.85 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | J., Stein. A., (2000) Acta Crystallogr., Sect.D, 56, 348. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | ammonium acetate | 20 (mM) | |
| 3 | 1 | drop | Tris | 10 (mM) | |
| 4 | 1 | drop | beta-mercaptoethanol | 10 (mM) | |
| 5 | 1 | reservoir | PEG8000 | 2-5 (%(v/v)) | |
| 6 | 1 | reservoir | sodium acetate | 100 (mM) | pH4.5 |
