1JK9
Heterodimer between H48F-ySOD1 and yCCS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 5ID-B |
Synchrotron site | APS |
Beamline | 5ID-B |
Temperature [K] | 113 |
Detector technology | CCD |
Collection date | 2001-02-08 |
Detector | MARRESEARCH |
Wavelength(s) | 1.0 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 104.090, 104.090, 233.710 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 12.000 - 2.900 |
R-factor | 0.217 |
Rwork | 0.217 |
R-free | 0.26000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | heterodimer |
RMSD bond length | 0.007 * |
RMSD bond angle | 1.400 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 12.000 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.109 * | 0.351 * |
Total number of observations | 119535 * | |
Number of reflections | 32933 | |
Completeness [%] | 98.8 | 99.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 25 * | MES, ammonium sulfate, dioxane, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 40 (mg/ml) | |
2 | 1 | drop | MES | 50 (mM) | |
3 | 1 | drop | 150 (mM) | ||
4 | 1 | drop | dithiothreitol | 20 (mM) | |
5 | 1 | drop | 0.020 (mM) | ||
6 | 1 | reservoir | MES | 100 (mM) | |
7 | 1 | reservoir | ammonium sulfate | 1.8 (M) | |
8 | 1 | reservoir | dioxane | 5 (%) |