1JK4

DES 1-6 BOVINE NEUROPHYSIN II COMPLEX WITH VASOPRESSIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	1996-07-01
Detector	MARRESEARCH
Spacegroup name	P 61 2 2
Unit cell lengths	50.900, 50.900, 105.800
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	15.000 - 2.300
R-factor	0.234
Rwork	0.234
R-free	0.29900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bn2
RMSD bond length	0.010
RMSD bond angle	26.600 *
Data reduction software	X-GEN
Data scaling software	X-GEN
Phasing software	AMoRE
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	999.000
High resolution limit [Å]	2.300
Rmerge	0.058
Completeness [%]	96.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5 *	291 *	Wu, C.K., (1996) Acta Cryst., D52, 946. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	14 (mM)	in water
2	1	drop	sodium azide	0.25 (mg/ml)
3	1	reservoir		2.0 (M)
4	1	reservoir	MES	0.1 (M)	pH6.5
5	1	reservoir	sodium/pootassium phosphate	0.05 (M)