1JK2
Zif268 D20A mutant bound to the GCT DNA site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 125 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-05-28 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 43.600, 56.050, 131.560 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.650 |
| R-factor | 0.23 |
| Rwork | 0.230 |
| R-free | 0.27300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | D20A bound to the wt site with the appropriate change to the DNA and with waters and side chains of residues 18 and 21 removed |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.052 | |
| Total number of observations | 138650 * | |
| Number of reflections | 18149 | |
| Completeness [%] | 91.3 | 75.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 * | 298 | Elrod-Erickson, M., (1998) Structure, 6,451. * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG1450 | ||
| 2 | 1 | 1 | Bis-Tris Propane | ||
| 3 | 1 | 1 | NaCl |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | 200 (mM) | ||
| 2 | 1 | drop | MES | 50 (mM) | |
| 3 | 1 | reservoir | 500 (mM) | ||
| 4 | 1 | reservoir | PEG1450 | 15 (%) | |
| 5 | 1 | reservoir | Bis-Tris-propane | 25 (mM) | pH8.0 |
