1JK0

Ribonucleotide reductase Y2Y4 heterodimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	2000-06-30
Detector	MARRESEARCH
Wavelength(s)	0.946
Spacegroup name	P 21 21 21
Unit cell lengths	92.900, 93.000, 97.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	9.000 - 2.800
R-factor	0.243
Rwork	0.243
R-free	0.29600
Structure solution method	MIR
RMSD bond length	0.008
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS (DERIVED MIR PHASES WITH SITES FOUND IN SOLVE)
Refinement software	CNS (derived MIR phases with sites found in SOLVE)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000 *	2.870
High resolution limit [Å]	2.800	2.800
Rmerge	0.063 *	0.285 *
Total number of observations	65155 *
Number of reflections	20645 *
<I/σ(I)>	17.7	2.7
Completeness [%]	99.9 *	99.8 *
Redundancy	3.1	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	sandwich drop	5.3 *	293	100 mM acetate, 14% w/v polyethyleneglycol 4000, 200 mM NaCl, pH 4.9, sandwich drop, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	Tris	50 (mM)
2	1	1	protein	4 (mg/ml)
3	1	2	acetate	100 (mM)
4	1	2	PEG4000	14 (%(w/v))
5	1	2		200 (mM)