1JJW
Structure of Haemophilus influenzae HslV Protein at 1.9 A Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-01-06 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.55 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 78.893, 122.331, 126.288 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 * - 1.900 |
| R-factor | 0.217 * |
| Rwork | 0.215 |
| R-free | 0.25100 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g3k |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.049 | 0.195 |
| Number of reflections | 46472 | |
| <I/σ(I)> | 28.8 | 9.1 |
| Completeness [%] | 96.4 | 98.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 277 | sodium citrate, HCl Tris, PEG 400, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | 5 (mM) | ||
| 3 | 1 | drop | ADP | 1 (mM) | |
| 4 | 1 | drop | MOPS | 10 (mM) | pH7.0 |
| 5 | 1 | reservoir | PEG400 | 30 (%) | |
| 6 | 1 | reservoir | sodium citrate | 100 (mM) | |
| 7 | 1 | reservoir | Tris-HCl | 100 (mM) | pH8.5 |
