1JJH
E2 DNA-binding Domain from Bovine Papillomavirus Type 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 103 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-06-01 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 122.290, 122.290, 85.019 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.500 |
| R-factor | 0.225 |
| Rwork | 0.225 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.048 | 0.307 |
| <I/σ(I)> | 16 | 2.14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 298 | ammonium sulfate, imidazole, magnesium chloride, glycerol, DTT, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6 (mg/ml) | |
| 10 | 1 | reservoir | glycerol | 10 (%) | |
| 11 | 1 | reservoir | dithiothreitol | 10 (mM) | |
| 2 | 1 | drop | ammonium sulfate | 0.5-0.6 (M) | |
| 3 | 1 | drop | imidazole | 25 (mM) | |
| 4 | 1 | drop | 1 (mM) | ||
| 5 | 1 | drop | glycerol | 5 (%(v/v)) | |
| 6 | 1 | drop | dithiothreitol | 5 (mg/ml) | |
| 7 | 1 | reservoir | ammonium sulfate | 1.1-1.3 (M) | |
| 8 | 1 | reservoir | imidazole | 50 (mM) | |
| 9 | 1 | reservoir | 2 (mM) |
