1JJH
E2 DNA-binding Domain from Bovine Papillomavirus Type 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 103 |
Detector technology | IMAGE PLATE |
Collection date | 1997-06-01 |
Detector | RIGAKU RAXIS IIC |
Wavelength(s) | 1.5418 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 122.290, 122.290, 85.019 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 2.500 |
R-factor | 0.225 |
Rwork | 0.225 |
R-free | 0.26600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (0.5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.540 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.048 | 0.307 |
<I/σ(I)> | 16 | 2.14 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 298 | ammonium sulfate, imidazole, magnesium chloride, glycerol, DTT, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 6 (mg/ml) | |
10 | 1 | reservoir | glycerol | 10 (%) | |
11 | 1 | reservoir | dithiothreitol | 10 (mM) | |
2 | 1 | drop | ammonium sulfate | 0.5-0.6 (M) | |
3 | 1 | drop | imidazole | 25 (mM) | |
4 | 1 | drop | 1 (mM) | ||
5 | 1 | drop | glycerol | 5 (%(v/v)) | |
6 | 1 | drop | dithiothreitol | 5 (mg/ml) | |
7 | 1 | reservoir | ammonium sulfate | 1.1-1.3 (M) | |
8 | 1 | reservoir | imidazole | 50 (mM) | |
9 | 1 | reservoir | 2 (mM) |