1JJA
CRYSTAL STRUCTURE OF ORTHORHOMBIC FORM OF D90E MUTANT OF ESCHERICHIA COLI L-ASPARAGINASE II
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | SIEMENS |
Temperature [K] | 290 |
Detector technology | IMAGE PLATE |
Collection date | 1998-09-10 |
Detector | MARRESEARCH |
Wavelength(s) | 1.54178 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 225.419, 128.049, 62.567 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.300 |
R-factor | 0.187 |
Rwork | 0.186 |
R-free | 0.20416 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Active dimer of native E.coli asparaginase II (PDB code: 3ECA) |
RMSD bond length | 0.014 |
RMSD bond angle | 0.025 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | EPMR |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.410 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.123 | 0.813 |
Number of reflections | 75961 | |
<I/σ(I)> | 11.2 | 1.8 |
Completeness [%] | 93.9 | |
Redundancy | 3.14 | 3.07 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 292 | PEG MME550, bicine, NaCl , pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K |