1JJ1

CRYSTAL STRUCTURE OF ORTHORHOMBIC LYSOZYME GROWN AT PH 4.6 IN PRESENCE OF 5% SORBITOL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	30.558, 58.995, 68.258
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.900
R-factor	0.188 *
Rwork	0.186
R-free	0.22300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	193l
RMSD bond length	0.005 *
RMSD bond angle	1.400
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.081 *	0.391 *
Total number of observations	41051 *
Number of reflections	11864	1089 *
<I/σ(I)>	8.1
Completeness [%]	98.8	94.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	4.6	293	Na-acetate buffer, pH 4.6, 10% NaCl, 5% sorbitol, LIQUID DIFFUSION at 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	acetate	0.04 (M)	pH4.6