1JIV
T4 phage BGT in complex with Mg2+ : Form II
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-02-24 |
Detector | MARRESEARCH |
Wavelength(s) | 0.934 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.724, 72.749, 79.661 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.070 |
R-factor | 0.186 * |
Rwork | 0.186 |
R-free | 0.23700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qkj |
RMSD bond length | 0.006 |
RMSD bond angle | 1.250 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.140 |
High resolution limit [Å] | 2.070 | 2.070 |
Rmerge | 0.088 | 0.243 |
Total number of observations | 381698 * | |
Number of reflections | 23440 | |
<I/σ(I)> | 10.7 | 4.8 |
Completeness [%] | 99.4 | 99.2 |
Redundancy | 16.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | PEG 4000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | PEG4000 | 10-11 (%) | |
2 | 1 | drop | Tris-HCl | 100 (mM) | pH7.5 |
3 | 1 | drop | UDP | 1 (mM) | |
4 | 1 | drop | 40 (mM) | ||
5 | 1 | reservoir | PEG4000 | 20-22 (%) |