1JIF

Crystal structure of bleomycin-binding protein from bleomycin-producing Streptomyces verticillus complexed with copper(II)-bleomycin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	2000-04-01
Detector	RIGAKU RAXIS IIC
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	35.730, 83.470, 43.060
Unit cell angles	90.00, 106.20, 90.00

Refinement procedure

Resolution	10.000 - 1.600
R-factor	0.159 *
R-free	0.20800 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015 *
RMSD bond angle	0.038 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	1.700
High resolution limit [Å]	1.600	1.600
Rmerge	0.059	0.242
Number of reflections	30741
<I/σ(I)>	18.4	1.98
Completeness [%]	96.7	84.5
Redundancy	4.29

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	25 *	PEG4000, ammonium acetate, sodium cacodylate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	30.0 (%(w/v))
2	1	reservoir	ammonium acetate	0.2 (M)
3	1	reservoir	sodium cacodylate	0.1 (M)	pH6.0