1JID
Human SRP19 in complex with helix 6 of Human SRP RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-05-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.91902 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 55.813, 109.782, 89.461 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.750 - 1.800 |
| R-factor | 0.186 * |
| Rwork | 0.186 |
| R-free | 0.22200 |
| Structure solution method | SAD with molecular replacement |
| Starting model (for MR) | 1d4r |
| RMSD bond length | 0.017 |
| RMSD bond angle | 3.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.750 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.088 | 0.197 |
| Number of reflections | 23784 | |
| <I/σ(I)> | 11.4 | 2.7 |
| Completeness [%] | 91.5 | 61.2 |
| Redundancy | 7.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 18 * | potassium chloride, magnesium chloride, sodium chloride, PEG 3350, isopropanol, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | KCl | ||
| 2 | 1 | 1 | MgCl2 | ||
| 3 | 1 | 1 | NaCl | ||
| 4 | 1 | 1 | PEG 3350 | ||
| 5 | 1 | 1 | isopropanol |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | sodium citrate | 50 (mM) | |
| 3 | 1 | reservoir | 450 (mM) | ||
| 4 | 1 | reservoir | PEG3350 | 10 (%) |
