1JI7
Crystal Structure of TEL SAM Polymer
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 2000-04-10 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.978803, 0.971349, 0.979018 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 132.865, 52.678, 58.701 |
| Unit cell angles | 90.00, 113.90, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.450 |
| Rwork | 0.184 |
| R-free | 0.19500 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.170 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.900 | 1.510 |
| High resolution limit [Å] | 1.400 * | 1.400 * |
| Rmerge | 0.057 | 0.383 * |
| Total number of observations | 244968 * | |
| Number of reflections | 139887 | |
| <I/σ(I)> | 12.2 | 2.6 |
| Completeness [%] | 97.4 | 97.3 * |
| Redundancy | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 * | 298 | Li2SO4, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7 (mg/ml) | |
| 2 | 1 | drop | Tris | 10 (mM) | pH8.5 |
| 3 | 1 | drop | 200 (mM) | ||
| 4 | 1 | reservoir | Tris | 50 (mM) | pH8.0 |
| 5 | 1 | reservoir | 1.6 (M) |
