1JI4
NAP protein from helicobacter pylori
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-07-12 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.3 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 88.778, 133.225, 95.228 |
| Unit cell angles | 90.00, 93.99, 90.00 |
Refinement procedure
| Resolution | 20.810 - 2.520 |
| Rwork | 0.215 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qgh |
| RMSD bond length | 0.008 |
| RMSD bond angle | 19.900 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.660 |
| High resolution limit [Å] | 2.500 | 2.520 * |
| Rmerge | 0.045 | 0.087 |
| Number of reflections | 71729 | 9698 * |
| <I/σ(I)> | 11.3 | 5.9 |
| Completeness [%] | 96.7 | 90.9 |
| Redundancy | 2.9 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | ammonium acetate, citrate buffer, MPD, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium acetate | 0.2 (M) | |
| 2 | 1 | reservoir | citrate | 0.1 (M) | pH5.6 |
| 3 | 1 | reservoir | MPD | 30 (%(v/v)) |
