1JHZ
Purine Repressor Mutant Corepressor Binding Domain Structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-07-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.08 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.020, 126.130, 61.840 |
| Unit cell angles | 90.00, 99.71, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.400 |
| R-factor | 0.178 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dbq |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.018 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | TNT |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 2.460 | |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.079 | 0.369 |
| Number of reflections | 20934 | |
| <I/σ(I)> | 6.64 | 1.9 |
| Completeness [%] | 92.2 | 93.5 |
| Redundancy | 3 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 298 | PEG 600, magnesium chloride, Tris HCl, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | PEG600 | 20 (%) | |
| 3 | 1 | reservoir | 0.2 (M) | ||
| 4 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH7.6 |
