1JHS
Protein Mog1 E65A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-04-24 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9330 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.365, 48.972, 111.976 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.200 * - 1.900 |
| Rwork | 0.208 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Mog1 native |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.460 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.200 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.046 | 0.076 |
| Total number of observations | 62794 * | |
| Number of reflections | 18191 | |
| <I/σ(I)> | 6.4 | 8.9 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 3.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 * | 291 | Baker, R.P., (2000) Acta Crystallogr., D56, 229. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 16-20 (%) | |
| 2 | 1 | reservoir | glycerol | 20 (%) | |
| 3 | 1 | reservoir | dithiothreitol | 10 (mM) | |
| 4 | 1 | reservoir | acetate | or MES, pH6.0-6.5 | |
| 5 | 1 | reservoir | beta-octylglucoside | 0.1-0.25 (%) | |
| 6 | 1 | drop | protein | 10-12 (mg/ml) | |
| 7 | 1 | drop | ammonium acetate | 0.2 (M) | |
| 8 | 1 | drop | sodium citrate | 1 (M) | pH5.6 |
| 9 | 1 | drop | PEG4000 | 30 (%) |
