1JHJ
Crystal structure of the APC10/Doc1 subunit of the human anaphase-promoting complex
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-09-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9791, 0.9797, 0.9500, 1.4800, 1.600 |
| Spacegroup name | P 6 2 2 |
| Unit cell lengths | 97.664, 97.664, 67.769 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 500.000 * - 1.600 |
| R-factor | 0.209 * |
| Rwork | 0.209 |
| R-free | 0.24400 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 * |
| RMSD bond angle | 1.711 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.047 | |
| <I/σ(I)> | 35.86 | |
| Completeness [%] | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 18 * | ammonium sulfate, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 1.9 (M) | |
| 2 | 1 | reservoir | HEPES | 0.1 (M) | |
| 3 | 1 | reservoir | 0.1 (M) | ||
| 4 | 1 | reservoir | nickel acetate | 5 (mM) | |
| 5 | 1 | reservoir | aniline | 5 (%) |
