1JHG
TRP REPRESSOR MUTANT V58I
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 287 |
| Detector technology | IMAGE PLATE AREA DETECTOR |
| Collection date | 1994-08 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 2 21 |
| Unit cell lengths | 53.536, 32.789, 53.385 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.300 |
| R-factor | 0.1218 |
| R-free | 0.17200 |
| Structure solution method | ISOMORPHOUS TO PDB ENTRY 2WRP |
| RMSD bond length | 0.017 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXL-93 |
| Refinement software | SHELXL-93 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.350 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.032 | 0.167 |
| Number of reflections | 86193 | |
| <I/σ(I)> | 28.4 | 20 |
| Completeness [%] | 97.4 | 94 |
| Redundancy | 3.6 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | HANGING DROP VAPOR DIFFUSION 2.0 M SODIUM PHOSPHATE, PH 5.0 600 MM AMMONIUM CHLORIDE 5 MM L-TRYPTOPHAN, vapor diffusion - hanging drop |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | sodium phosphate | 2.0 (M) | |
| 2 | 1 | reservoir | ammonium chloride | 600 (mM) | |
| 3 | 1 | reservoir | L-trp | 1-5 (mM) |
