1JH5
Crystal Structure of sTALL-1 of TNF family ligand
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 234.242, 234.242, 212.550 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.990 - 3.000 |
| R-factor | 0.236 |
| Rwork | 0.236 |
| R-free | 0.25200 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.600 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 3.000 |
| Rmerge | 0.117 |
| Total number of observations | 334821 * |
| Number of reflections | 66001 |
| <I/σ(I)> | 12 |
| Completeness [%] | 95.7 |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 9 | 277 | 35% dioxane, pH 9.0, VAPOR DIFFUSION, HANGING DROP at 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | reservoir | beta-mercaptoethanol | 5 (mM) | |
| 3 | 1 | reservoir | Bicine | 50 (mM) | pH9.0 |
| 4 | 1 | reservoir | 150 (mM) | ||
| 5 | 1 | reservoir | dioxane | 35-38 (%) |
