1JH0
Photosynthetic Reaction Center Mutant With Glu L 205 Replaced to Leu
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-02-04 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 141.800, 141.800, 187.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.900 - 3.500 |
| R-factor | 0.216 * |
| Rwork | 0.225 |
| R-free | 0.26100 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qov |
| RMSD bond length | 0.008 * |
| RMSD bond angle | 1.570 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.620 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.232 | 0.281 * |
| Total number of observations | 114799 * | |
| Number of reflections | 26839 | |
| <I/σ(I)> | 5.3 | |
| Completeness [%] | 95.9 | 93.6 * |
| Redundancy | 7.94 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.5 * | 298 | Potasium phosphate, LDAO, heptane triol, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | LDAO | 0.08 (%) | |
| 3 | 1 | drop | potassium phosphate | 0.75 (M) | |
| 4 | 1 | drop | heptanetriol | 3.5 (%) | |
| 5 | 1 | reservoir | potassium phosphate | 1.6 (M) |
