1JH0

Photosynthetic Reaction Center Mutant With Glu L 205 Replaced to Leu

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1999-02-04
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 31 2 1
Unit cell lengths	141.800, 141.800, 187.400
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.900 - 3.500
R-factor	0.216 *
Rwork	0.225
R-free	0.26100 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qov
RMSD bond length	0.008 *
RMSD bond angle	1.570 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.620
High resolution limit [Å]	3.500	3.500
Rmerge	0.232	0.281 *
Total number of observations	114799 *
Number of reflections	26839
<I/σ(I)>	5.3
Completeness [%]	95.9	93.6 *
Redundancy	7.94	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.5 *	298	Potasium phosphate, LDAO, heptane triol, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	LDAO	0.08 (%)
3	1	drop	potassium phosphate	0.75 (M)
4	1	drop	heptanetriol	3.5 (%)
5	1	reservoir	potassium phosphate	1.6 (M)