1JGY
Photosynthetic Reaction Center Mutant With Tyr M 76 Replaced With Phe
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-04-11 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.08 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 141.680, 141.680, 186.840 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.100 - 2.700 |
| R-factor | 0.212 * |
| Rwork | 0.218 |
| R-free | 0.25200 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qov |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.310 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.103 | 0.210 |
| Total number of observations | 75641 * | |
| Number of reflections | 35678 | |
| Completeness [%] | 59.5 | 38.7 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.5 * | 298 | Potasium phosphate, LDAO, heptane triol, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | LDAO | 0.08 (%) | |
| 3 | 1 | drop | potassium phosphate | 0.75 (M) | |
| 4 | 1 | drop | heptanetriol | 3.5 (%) | |
| 5 | 1 | reservoir | potassium phosphate | 1.6 (M) |
