1JGD
HLA-B*2709 bound to deca-peptide s10R
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-08-21 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0500 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 129.844, 48.438, 88.532 |
| Unit cell angles | 90.00, 121.29, 90.00 |
Refinement procedure
| Resolution | 14.000 * - 1.900 |
| R-factor | 0.17 |
| Rwork | 0.169 |
| R-free | 0.19400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jge |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.900 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.056 | 0.386 |
| Number of reflections | 37162 | 1849 * |
| <I/σ(I)> | 28.8 | 4.6 |
| Completeness [%] | 99.6 * | 98.6 |
| Redundancy | 6 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | PEG 8000, Tris-HCl, NaCl; glycerol as cryo protectant, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
