1JGC
The 2.6 A Structure Resolution of Rhodobacter capsulatus Bacterioferritin with Metal-free Dinuclear Site and Heme Iron in a Crystallographic Special Position
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2000-02-17 |
Detector | MARRESEARCH |
Wavelength(s) | 1.08 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 142.370, 142.370, 140.880 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 21.500 * - 2.600 |
R-factor | 0.225 * |
Rwork | 0.225 |
R-free | 0.24200 |
Structure solution method | MIR |
RMSD bond length | 0.008 |
RMSD bond angle | 17.990 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 21.500 * | 2.640 |
High resolution limit [Å] | 2.600 * | 2.590 |
Rmerge | 0.077 * | 0.331 * |
Total number of observations | 260696 * | |
Number of reflections | 22679 | |
<I/σ(I)> | 12.6 | |
Completeness [%] | 99.4 | 94 |
Redundancy | 11.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 4.6 | 293 | octyl glucoside, NaOAc, PEK 4K, pH 4.6, VAPOR DIFFUSION, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | octyl glucoside | 20 (mg/ml) | |
2 | 1 | reservoir | 0.1 (M) | pH4.6 | |
3 | 1 | reservoir | PEG4000 | 4 (%) |