1JG9
Crystal Structure of Amylosucrase from Neisseria polysaccharea in Complex with D-glucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 1999-12-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 96.131, 116.733, 61.295 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 12.980 - 1.660 |
| R-factor | 0.185 * |
| Rwork | 0.185 |
| R-free | 0.20400 |
| RMSD bond length | 0.008 * |
| RMSD bond angle | 1.600 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 13.000 | 1.720 |
| High resolution limit [Å] | 1.660 | 1.660 |
| Rmerge | 0.069 | 0.076 |
| Total number of observations | 619347 * | |
| Number of reflections | 81373 * | |
| <I/σ(I)> | 9 | 2.5 |
| Completeness [%] | 99.3 | 99.4 |
| Redundancy | 7 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 * | 4 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4-5 (mg/ml) | |
| 2 | 1 | drop | 150 (mM) | ||
| 3 | 1 | drop | Tris-HCl | 50 (mM) | |
| 4 | 1 | drop | EDTA | 1 (mM) | |
| 5 | 1 | drop | alpha-dithiothreitol | 1 (mM) | |
| 6 | 1 | reservoir | PEG6000 | 30 (%(w/v)) | |
| 7 | 1 | reservoir | HEPES | 100 (mM) |
