1JG9

Crystal Structure of Amylosucrase from Neisseria polysaccharea in Complex with D-glucose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	120
Detector technology	CCD
Collection date	1999-12-10
Detector	MARRESEARCH
Wavelength(s)	0.933
Spacegroup name	P 21 21 2
Unit cell lengths	96.131, 116.733, 61.295
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	12.980 - 1.660
R-factor	0.185 *
Rwork	0.185
R-free	0.20400
RMSD bond length	0.008 *
RMSD bond angle	1.600 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	13.000	1.720
High resolution limit [Å]	1.660	1.660
Rmerge	0.069	0.076
Total number of observations	619347 *
Number of reflections	81373 *
<I/σ(I)>	9	2.5
Completeness [%]	99.3	99.4
Redundancy	7	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	4-5 (mg/ml)
2	1	drop		150 (mM)
3	1	drop	Tris-HCl	50 (mM)
4	1	drop	EDTA	1 (mM)
5	1	drop	alpha-dithiothreitol	1 (mM)
6	1	reservoir	PEG6000	30 (%(w/v))
7	1	reservoir	HEPES	100 (mM)