1JFR
CRYSTAL STRUCTURE OF THE STREPTOMYCES EXFOLIATUS LIPASE AT 1.9A RESOLUTION: A MODEL FOR A FAMILY OF PLATELET-ACTIVATING FACTOR ACETYLHYDROLASES
Experimental procedure
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS FR591 |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 1996-07-08 |
Detector | MACSCIENCE |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 77.500, 53.000, 81.100 |
Unit cell angles | 90.00, 95.80, 90.00 |
Refinement procedure
Resolution | 8.000 - 1.900 |
R-factor | 0.14 * |
Rwork | 0.140 |
R-free | 0.18600 |
Structure solution method | MIR |
RMSD bond length | 0.016 |
RMSD bond angle | 0.028 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELX-90 |
Refinement software | SHELX-90 |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 1.900 |
Rmerge | 0.054 * |
Total number of observations | 235302 * |
Number of reflections | 49042 |
<I/σ(I)> | 14.75 |
Completeness [%] | 95.4 |
Redundancy | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7 * | pH 7.75 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | Tris-HCl | 50 (mM) | |
2 | 1 | drop | protein | 5.0-7.0 (mg/ml) | |
3 | 1 | reservoir | ammonium sulfate | 30 (%) | |
4 | 1 | reservoir | MME2000 | 10 (%) | |
5 | 1 | reservoir | PEG6000 | 6 (%) | |
6 | 1 | reservoir | phosphate | 200 (mM) |