1JFR
CRYSTAL STRUCTURE OF THE STREPTOMYCES EXFOLIATUS LIPASE AT 1.9A RESOLUTION: A MODEL FOR A FAMILY OF PLATELET-ACTIVATING FACTOR ACETYLHYDROLASES
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-07-08 |
| Detector | MACSCIENCE |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 77.500, 53.000, 81.100 |
| Unit cell angles | 90.00, 95.80, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.900 |
| R-factor | 0.14 * |
| Rwork | 0.140 |
| R-free | 0.18600 |
| Structure solution method | MIR |
| RMSD bond length | 0.016 |
| RMSD bond angle | 0.028 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX-90 |
| Refinement software | SHELX-90 |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 1.900 |
| Rmerge | 0.054 * |
| Total number of observations | 235302 * |
| Number of reflections | 49042 |
| <I/σ(I)> | 14.75 |
| Completeness [%] | 95.4 |
| Redundancy | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 * | pH 7.75 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris-HCl | 50 (mM) | |
| 2 | 1 | drop | protein | 5.0-7.0 (mg/ml) | |
| 3 | 1 | reservoir | ammonium sulfate | 30 (%) | |
| 4 | 1 | reservoir | MME2000 | 10 (%) | |
| 5 | 1 | reservoir | PEG6000 | 6 (%) | |
| 6 | 1 | reservoir | phosphate | 200 (mM) |
