1JFH

STRUCTURE OF A PANCREATIC ALPHA-AMYLASE BOUND TO A SUBSTRATE ANALOGUE AT 2.03 ANGSTROM RESOLUTION

Experimental procedure

Detector technology	IMAGE PLATE
Detector	MAR scanner 180 mm plate
Spacegroup name	P 21 21 21
Unit cell lengths	56.300, 87.800, 103.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.000 - 2.030
R-factor	0.16
Rwork	0.160
R-free	0.18500
RMSD bond length	0.010
RMSD bond angle	1.400
Data reduction software	DENZO
Phasing software	X-PLOR (3.843)
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.030 *
Rmerge	0.057	0.139 *
Total number of observations	322368 *
Number of reflections	33718
Completeness [%]	99.4	99.8 *
Redundancy	9.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *