1JFF
Refined structure of alpha-beta tubulin from zinc-induced sheets stabilized with taxol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ELECTRON MICROSCOPE |
| Source details | JEOL 4000 electron microscope |
| Temperature [K] | 93 |
| Detector technology | FILM |
| Collection date | 1994-01-01 |
| Detector | KODAK |
| Wavelength(s) | 0.0194 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.200, 93.500, 90.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.500 |
| R-factor | 0.231 * |
| Rwork | 0.232 |
| R-free | 0.29660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tub |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.561 |
| Phasing software | CCP4 |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.700 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.250 | |
| Number of reflections | 12422 | |
| <I/σ(I)> | 5.4 | 2.3 |
| Completeness [%] | 73.0 | 73 |
| Redundancy | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 5.8 | 305 | Zn++, pH 5.8, aberrant polymerization of tubulin, temperature 305K |
| 1 | unknown * | 5.8 | 305 | Zn++, pH 5.8, aberrant polymerization of tubulin, temperature 305K |
