1JF3

Crystal Structure Of Component III Glycera Dibranchiata Monomeric Hemoglobin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	2001-04-05
Detector	RIGAKU RAXIS IIC
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	61.133, 32.792, 40.949
Unit cell angles	90.00, 109.33, 90.00

Refinement procedure

Resolution	10.000 - 1.800 *
R-factor	0.188 *
Rwork	0.189
R-free	0.22450 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	2.170 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000	30.000
High resolution limit [Å]	1.800 *	1.400
Rmerge	0.052 *	0.045
Number of reflections	9985 *
Completeness [%]	87.0 *	35 *
Redundancy	3.5	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.5	277	used microseeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	reservoir	PEG8000	15 (%)
3	1	reservoir	sodium cacodylate	50 (mM)
4	1	reservoir	ammonium sulfate	0.1 (M)	pH6.5