1JF2
Crystal Structure of W92F obelin mutant from Obelia longissima at 1.72 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-04-08 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 53.449, 53.449, 144.485 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.280 - 1.720 |
| R-factor | 0.232 |
| Rwork | 0.232 |
| R-free | 0.27400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1el4 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.037 * | 0.080 * |
| Number of reflections | 21633 | |
| <I/σ(I)> | 18.08 | |
| Completeness [%] | 99.9 * | 87.7 |
| Redundancy | 5.8 * | 10.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 4 * | Deng, L., (2001) FEBS Lett., 506, 281. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8-10 (mg/ml) | |
| 2 | 1 | reservoir | PEG8000 | 18 (%) | |
| 3 | 1 | reservoir | potassium phosphate | 50 (mM) | |
| 4 | 1 | drop | hexamine cobalt chloride |
