1JF0
The Crystal Structure of Obelin from Obelia geniculata at 1.82 A Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-09-03 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 33.502, 67.823, 69.209 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.630 - 1.820 |
| R-factor | 0.212 |
| Rwork | 0.212 |
| R-free | 0.26200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1el4 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.100 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.000 | 1.860 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rmerge | 0.035 | 0.219 |
| Number of reflections | 14015 | |
| <I/σ(I)> | 28.19 | 3.8 |
| Completeness [%] | 95.1 | 93.9 |
| Redundancy | 12 | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 277 | PEG 8000, potassium phosphate, hexaminecobaltic chloride, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
