1JEQ
Crystal Structure of the Ku Heterodimer
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-10-23 |
| Detector | BRANDEIS - B4 |
| Wavelength(s) | 0.9633, 0.9786, 0.9791 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 80.209, 86.186, 203.277 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 2.700 |
| R-factor | 0.222 * |
| Rwork | 0.222 |
| R-free | 0.28400 * |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.430 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.080 * | 0.410 |
| Total number of observations | 94522 * | |
| Number of reflections | 35715 * | |
| <I/σ(I)> | 8.2 | |
| Completeness [%] | 92.9 | 69.8 |
| Redundancy | 5.2 | 2.82 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 22 * | 14% PEG 4000, 900 mM sarcosine, 140 mM magnesium chloride, 100 mM Tris-HCl (pH 8.5), VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 18 (mg/ml) | |
| 2 | 1 | reservoir | PEG350MME | 26 (%) | |
| 3 | 1 | reservoir | Tris-HCl | 50 (mM) | pH8.0 |
| 4 | 1 | reservoir | 5 (mM) |
