1JEQ
Crystal Structure of the Ku Heterodimer
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-10-23 |
Detector | BRANDEIS - B4 |
Wavelength(s) | 0.9633, 0.9786, 0.9791 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 80.209, 86.186, 203.277 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 * - 2.700 |
R-factor | 0.222 * |
Rwork | 0.222 |
R-free | 0.28400 * |
Structure solution method | MAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.430 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.080 * | 0.410 |
Total number of observations | 94522 * | |
Number of reflections | 35715 * | |
<I/σ(I)> | 8.2 | |
Completeness [%] | 92.9 | 69.8 |
Redundancy | 5.2 | 2.82 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 22 * | 14% PEG 4000, 900 mM sarcosine, 140 mM magnesium chloride, 100 mM Tris-HCl (pH 8.5), VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 18 (mg/ml) | |
2 | 1 | reservoir | PEG350MME | 26 (%) | |
3 | 1 | reservoir | Tris-HCl | 50 (mM) | pH8.0 |
4 | 1 | reservoir | 5 (mM) |