1JEK
Visna TM CORE STRUCTURE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-12-01 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 52.548, 52.548, 61.686 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 9.960 - 1.500 |
| R-factor | 0.213 |
| Rwork | 0.213 |
| R-free | 0.26500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2siv |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.520 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.066 | 0.259 * |
| Total number of observations | 52353 * | |
| Number of reflections | 16300 | |
| <I/σ(I)> | 13.9 | 2.5 |
| Completeness [%] | 90.1 | 64.7 |
| Redundancy | 7.5 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 25% tert-butanol, 0.1M TRIS-HCL, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | tert-butanol | 25 (%) | |
| 3 | 1 | reservoir | Tris-HCl | 0.1 (M) |
