1JEK
Visna TM CORE STRUCTURE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1998-12-01 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 52.548, 52.548, 61.686 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 9.960 - 1.500 |
R-factor | 0.213 |
Rwork | 0.213 |
R-free | 0.26500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2siv |
RMSD bond length | 0.012 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.520 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.066 | 0.259 * |
Total number of observations | 52353 * | |
Number of reflections | 16300 | |
<I/σ(I)> | 13.9 | 2.5 |
Completeness [%] | 90.1 | 64.7 |
Redundancy | 7.5 | 1.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 25% tert-butanol, 0.1M TRIS-HCL, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | reservoir | tert-butanol | 25 (%) | |
3 | 1 | reservoir | Tris-HCl | 0.1 (M) |