1JEF
TURKEY LYSOZYME COMPLEX WITH (GLCNAC)3
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | ELLIOTT GX-21 |
| Temperature [K] | 293 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1990-02 |
| Detector | ENRAF-NONIUS FAST |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 87.490, 32.940, 49.000 |
| Unit cell angles | 90.00, 119.10, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.770 |
| R-factor | 0.182 |
| Rwork | 0.182 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | STRUCTURE IN THE NATIVE CRYSTAL |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.700 |
| Data reduction software | MERGEF |
| Data scaling software | MERGEF |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.400 | 1.790 |
| High resolution limit [Å] | 1.750 | 1.760 |
| Rmerge | 0.058 | 0.225 |
| Total number of observations | 50234 * | |
| Number of reflections | 10950 | |
| Completeness [%] | 86.0 | |
| Redundancy | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microdialysis * | 4.2 | Harata, K., (1993) Acta Crystallogr.,Sect.D, 49, 497. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | ammonium sulfate | 3 (M) | 5 ml |
| 2 | 1 | 1 | 1-propanol | 10 (%) | 2 ml |
| 3 | 1 | 1 | sulfric acid | 0.05 (M) | |
| 4 | 1 | 1 | protein | 100 mg |
