1JE6
Structure of the MHC Class I Homolog MICB
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-10-05 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.10, 0.97953, 0.9793, 0.9567 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 81.700, 81.700, 86.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.2588 |
| Rwork | 0.255 |
| R-free | 0.29800 * |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 25.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.620 | 0.353 |
| Number of reflections | 10656 | |
| <I/σ(I)> | 49.9 | 6.8 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 10.1 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 22 * | ammonium sulfate, Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10-15 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 1.2 (M) | |
| 3 | 1 | reservoir | Tris | 50 (mM) | pH8.5 |
