1JE5

Crystal Structure of gp2.5, a Single-Stranded DNA Binding Protein Encoded by Bacteriophage T7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	1999-01-19
Detector	ADSC QUANTUM 4
Wavelength(s)	0.95
Spacegroup name	P 21 21 21
Unit cell lengths	68.100, 71.800, 82.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.710 - 1.900
R-factor	0.214 *
Rwork	0.221
R-free	0.26800
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	1.780 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	25.000
High resolution limit [Å]	1.900
Rmerge	0.040
Number of reflections	32408
<I/σ(I)>	17
Completeness [%]	98.6 *
Redundancy	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7 *	22 *	PEG 4000, calcium chloride, ethylene glycol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
1	VAPOR DIFFUSION, HANGING DROP	7 *	22 *	PEG 4000, calcium chloride, ethylene glycol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12 (mg/ml)
2	1	reservoir	PEG4000	22 (%)
3	1	reservoir	Tris-Cl	100 (mM)
4	1	reservoir		250 (mM)
5	1	reservoir	ethylene glycol	14 (%)