1JDN

Crystal Structure of Hormone Receptor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2001-01-09
Detector	ADSC QUANTUM 4
Wavelength(s)	1.08
Spacegroup name	P 61 2 2
Unit cell lengths	217.189, 217.189, 130.793
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.900
R-factor	0.243 *
Rwork	0.243
R-free	0.25600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	The complex of this receptor with hormone being processed presently.
RMSD bond length	0.009
RMSD bond angle	1.400
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.000
High resolution limit [Å]	2.900	2.900
Rmerge	0.081	0.435 *
Total number of observations	217841 *
Number of reflections	43722
<I/σ(I)>	7.4	2
Completeness [%]	98.1	98.4
Redundancy	4.9	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	other *	7.5	298	potassium sodium phosphate, lithium chloride, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	sodium potassium phosphate	1.5 (M)	or potassium phosphate