1JDL
Structure of cytochrome c2 from Rhodospirillum Centenum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200HB |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 1999-10-10 |
Detector | RIGAKU RAXIS IIC |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 29.700, 59.900, 65.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.700 |
R-factor | 0.192 * |
Rwork | 0.190 |
R-free | 0.21000 |
Structure solution method | SAD, MIR,MOLECULAR REPLACEMENT |
Starting model (for MR) | 2c2c |
RMSD bond length | 0.005 * |
RMSD bond angle | 1.330 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 20.000 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.070 | 0.320 |
Total number of observations | 223519 * | |
Number of reflections | 13248 | 1169 * |
<I/σ(I)> | 2986 | 149 |
Completeness [%] | 98.6 | 88.4 |
Redundancy | 16.9 | 10 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 * | PEG 6000, potassium phosphate, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 * | PEG 6000, potassium phosphate, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 * | PEG 6000, potassium phosphate, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | reservoir | PEG6000 | 10 (%) | |
3 | 1 | reservoir | phosphate | 0.1 (M) | pH7 |