1JDI

CRYSTAL STRUCTURE OF L-RIBULOSE-5-PHOSPHATE 4-EPIMERASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Detector technology	CCD
Collection date	1999-07-25
Detector	BRANDEIS - B4
Wavelength(s)	0.97
Spacegroup name	P 4 21 2
Unit cell lengths	105.900, 105.900, 274.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.400
R-factor	0.208
Rwork	0.208
R-free	0.24100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fua
RMSD bond length	0.007
RMSD bond angle	1.262
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.056	0.201
Number of reflections	58293	4187 *
<I/σ(I)>	19.4	4.1
Completeness [%]	93.8	68.8
Redundancy	3.64 *	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	295	4.0 M sodium formate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	sodium formate	4.0 (M)
3	1	reservoir	n-octyl-beta-D-glucoside	0.1 (%)