1JDA

MALTOTETRAOSE-FORMING EXO-AMYLASE

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1993-12-03
Detector	RIGAKU
Spacegroup name	P 21 21 21
Unit cell lengths	65.700, 171.000, 46.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.200
R-factor	0.174 *
Rwork	0.174
RMSD bond length	0.020
RMSD bond angle	0.040
Phasing software	X-PLOR
Refinement software	PROFFT

Data quality characteristics

	Overall
High resolution limit [Å]	2.200 *
Rmerge	0.105
Total number of observations	39300 *
Number of reflections	17893
Completeness [%]	65.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *	20 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	2 (%(w/v))
2	1	drop	Tris-HCl	20 (mM)
3	1	drop	ammonium sulfate	0.9 (M)
4	1	reservoir	maltopentaose	10 (mg/ml)