1JD4

Crystal Structure of DIAP1-BIR2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-12-30
Detector	RIGAKU RAXIS IIC
Spacegroup name	I 4
Unit cell lengths	96.100, 96.100, 59.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.700
R-factor	0.236 *
Rwork	0.236
R-free	0.28900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1g73
RMSD bond length	0.008
RMSD bond angle	1.334
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.113	0.437
Total number of observations	36725 *
Number of reflections	7220
<I/σ(I)>	15
Completeness [%]	95.1	95.5
Redundancy	5	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	296	Tris, 2,4-methyl-pentanediol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 296.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	Tris	100 (mM)	pH8.0
3	1	reservoir	MPD	60 (%(v/v))
4	1	reservoir	dithiothreitol	10 (mM)