1JD1
Crystal Structure of YEO7_yeast
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 170 |
| Detector technology | CCD |
| Collection date | 2000-08-24 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.98091, 0.99104 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.180, 64.100, 80.850 |
| Unit cell angles | 90.00, 90.63, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.700 |
| R-factor | 0.207 * |
| Rwork | 0.207 |
| R-free | 0.23800 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.740 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.039 * | 0.333 * |
| Total number of observations | 1452497 * | |
| Number of reflections | 88185 * | |
| <I/σ(I)> | 30 | 4.4 |
| Completeness [%] | 98.7 * | 97.1 * |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | 20 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | 100 (mM) | ||
| 3 | 1 | drop | Tris-Cl | 20 (mM) | |
| 4 | 1 | drop | dithiothreitol | 10 (mM) | |
| 5 | 1 | reservoir | ammonium sulfate | 1.9 (M) | |
| 6 | 1 | reservoir | succinate | 100 (mM) |
