1JC7
The Laminin-Binding Domain of Agrin is Structurally Related to N-TIMP-1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, Hamburg |
Beamline | X11 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Wavelength(s) | 0.847 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 85.306, 49.899, 54.328 |
Unit cell angles | 90.00, 118.43, 90.00 |
Refinement procedure
Resolution | 27.920 - 2.730 |
R-factor | 0.207 |
Rwork | 0.207 |
R-free | 0.24900 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
High resolution limit [Å] | 2.700 * | |
Rmerge | 0.086 * | 0.272 * |
Completeness [%] | 99.1 * | 94.3 * |
Redundancy | 4.5 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 4 * | PEG4000, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 8 (mg/ml) | |
2 | 1 | drop | citrate | 0.1 (M) | |
3 | 1 | reservoir | citrate | 0.1 (M) | |
4 | 1 | reservoir | PEG4000 | 20 (%(w/v)) | |
5 | 1 | reservoir | 2-propanol | 10 (%(w/v)) | |
6 | 1 | reservoir | MPD | 10 (%(w/v)) |