1JBY
CRYSTAL STRUCTURE ANALYSIS OF A DUAL-WAVELENGTH EMISSION GREEN FLUORESCENT PROTEIN VARIANT AT LOW PH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-07-19 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.953, 62.244, 67.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.500 - 1.800 |
| Rwork | 0.194 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ema |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.100 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | TNT |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.500 | 1.890 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.062 | 0.273 |
| Total number of observations | 83420 * | |
| Number of reflections | 18903 | |
| <I/σ(I)> | 6.4 | 2.7 |
| Completeness [%] | 93.0 | 87.2 |
| Redundancy | 4.4 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.9 * | 295 | PEG 1550, citrate, ammonium acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 48 (mg/ml) | |
| 2 | 1 | drop | HEPES | 20 (mM) | pH7.9 |
| 3 | 1 | reservoir | citrate-NaOH | 100 (mM) | pH4.5 |
| 4 | 1 | reservoir | ammonium acetate | 100 (mM) | |
| 5 | 1 | reservoir | PEG1550 | 14 (%) | pH5.5 |
