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1JBT

CRYSTAL STRUCTURE OF RIBOTOXIN RESTRICTOCIN COMPLEXED WITH A 29-MER SARCIN/RICIN DOMAIN RNA ANALOG

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 14-BM-C
Synchrotron siteAPS
Beamline14-BM-C
Temperature [K]100
Detector technologyCCD
Collection date2001-05-27
DetectorADSC QUANTUM 4
Wavelength(s)1.00
Spacegroup nameP 1 21 1
Unit cell lengths62.001, 102.822, 41.617
Unit cell angles90.00, 93.88, 90.00
Refinement procedure
Resolution19.770 - 2.700
R-factor0.224

*

Rwork0.224
R-free0.28200
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1aqz
RMSD bond length0.007
RMSD bond angle1.300
Data reduction softwareDENZO
Data scaling softwareSCALA
Phasing softwareAMoRE
Refinement softwareCNS (1.0)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]500.0002.750
High resolution limit [Å]2.7002.700
Rmerge0.0710.448
Number of reflections14201
<I/σ(I)>20.22.8
Completeness [%]97.696.5
Redundancy3.12.9
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1Vapor diffusion

*

7.2

*

19

*

PEG5000, K.MES, KCl, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K
Crystallization Reagents
IDcrystal IDsolution IDreagent nameconcentrationdetails
111PEG5000
211K.MES
311KCl
Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropTris10 (mM)
21drop50 (mM)
31dropsodium-EDTA50 (mM)
41reservoirPEG5000 MME2-10 (%(w/v))
51reservoirpotassium-MES100 (mM)
61reservoir50 (mM)

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PDB entries from 2024-11-06

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