1JBR
Crystal Structure of the Ribotoxin Restrictocin and a 31-mer SRD RNA Inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-03-22 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.400, 109.700, 40.000 |
| Unit cell angles | 90.00, 97.80, 90.00 |
Refinement procedure
| Resolution | 19.960 - 2.150 |
| R-factor | 0.232 * |
| Rwork | 0.232 |
| R-free | 0.29000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1aqz |
| RMSD bond length | 0.006 |
| RMSD bond angle | 25.000 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 500.000 | 2.190 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.048 | 0.465 |
| Number of reflections | 24648 | |
| <I/σ(I)> | 24.2 | 2.37 |
| Completeness [%] | 96.6 | 92.4 |
| Redundancy | 2.66 | 2.16 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.2 * | 19 * | PEG5000, K-MES, KCl, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG5000 | ||
| 2 | 1 | 1 | K-MES | ||
| 3 | 1 | 1 | KCl |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris | 10 (mM) | |
| 2 | 1 | drop | 50 (mM) | ||
| 3 | 1 | drop | sodium-EDTA | 50 (mM) | |
| 4 | 1 | reservoir | PEG5000 MME | 2-10 (%(w/v)) | |
| 5 | 1 | reservoir | potassium-MES | 100 (mM) | |
| 6 | 1 | reservoir | 50 (mM) |
