1JBO

The 1.45A Three-Dimensional Structure of c-Phycocyanin from the Thermophylic Cyanobacterium Synechococcus elongatus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX14.1
Synchrotron site	SRS
Beamline	PX14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2000-07-04
Detector	ADSC QUANTUM 4
Wavelength(s)	1.244
Spacegroup name	H 3 2
Unit cell lengths	187.995, 187.995, 60.542
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	95.000 * - 1.450
Rwork	0.146
R-free	0.18800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1cpc
RMSD bond length	0.039
RMSD bond angle	4.632 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	95.000 *	1.530
High resolution limit [Å]	1.450	1.450
Rmerge	0.088	0.261
Total number of observations	485268 *
Number of reflections	71757	10410 *
<I/σ(I)>	5.7	2.5
Completeness [%]	99.7	99.7
Redundancy	6.8	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.1	20 *	Ammonium Sulphate, MES, pH 6.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	1.1 (M)
2	1	reservoir	MES	40 (mM)	pH6.1
3	1	drop	protein	20 (mg/ml)