1JBG
Crystal Structure of MtaN, the Bacillus subtilis Multidrug Transporter Activator, N-terminus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1999-12-13 |
Detector | MARRESEARCH |
Wavelength(s) | 1.08 |
Spacegroup name | I 21 21 21 |
Unit cell lengths | 49.382, 67.838, 115.005 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 * - 2.750 |
R-factor | 0.228 * |
Rwork | 0.228 |
R-free | 0.28670 |
Structure solution method | MAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.500 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.300 | 2.820 |
High resolution limit [Å] | 2.750 | 2.750 |
Rmerge | 0.072 | 0.271 |
Total number of observations | 42297 * | |
Number of reflections | 5199 | |
<I/σ(I)> | 7.9 | 2.7 |
Completeness [%] | 99.3 * | 99 |
Redundancy | 8.1 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | Godsey, M., (2000) Acta Crystallogr., D56, 1456. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 11 (mg/ml) | |
2 | 1 | reservoir | 3.77 (M) | ||
3 | 1 | reservoir | HEPES | 50 (mM) | pH7.0 |
4 | 1 | reservoir | 10 (mM) |