1JB9
Crystal Structure of The Ferredoxin:NADP+ Reductase From Maize Root AT 1.7 Angstroms
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-03-01 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 59.670, 59.670, 189.100 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 * - 1.700 |
| R-factor | 0.164 * |
| Rwork | 0.164 |
| R-free | 0.22300 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Modified 1FNB |
| RMSD bond length | 0.013 |
| RMSD bond angle | 17.910 * |
| Data scaling software | SCALEPACK |
| Phasing software | MRX |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 |
| High resolution limit [Å] | 1.700 * | 1.700 |
| Rmerge | 0.068 * | 0.364 * |
| Total number of observations | 246386 * | |
| Number of reflections | 43654 * | |
| <I/σ(I)> | 13.5 | 3 |
| Completeness [%] | 99.1 * | 97.6 * |
| Redundancy | 5.4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 298 | Peg 8000, Na Cacodylate, Mg Acetate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 25 (mg/ml) | |
| 2 | 1 | drop | HEPES | 10 (mM) | pH7.0 |
| 3 | 1 | reservoir | PEG8000 | 20 (%) | |
| 4 | 1 | reservoir | sodium cacodylate | 0.1 (M) | pH6.5 |
| 5 | 1 | reservoir | magnesium acetate | 0.2 (M) |
