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1JB7

DNA G-Quartets in a 1.86 A Resolution Structure of an Oxytricha nova Telomeric Protein-DNA Complex

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 14-BM-C
Synchrotron siteAPS
Beamline14-BM-C
Temperature [K]100
Detector technologyCCD
Collection date1999-05-01
DetectorADSC QUANTUM 4
Wavelength(s)1.0000
Spacegroup nameP 61 2 2
Unit cell lengths93.100, 93.100, 421.800
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution20.000 - 1.860
R-factor0.23

*

Rwork0.230
R-free0.24600
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1otc
RMSD bond length0.007
RMSD bond angle1.300
Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareCNS
Refinement softwareCNS
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]20.0001.930
High resolution limit [Å]1.8601.860
Rmerge0.0650.540
Total number of observations903239

*

Number of reflections92060
<I/σ(I)>16.92.2
Completeness [%]99.195.6
Redundancy9.86.3
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP6

*

282drop contains the same amount of reservoir solution except 50mM NaCl

*

Crystallization Reagents
IDcrystal IDsolution IDreagent nameconcentrationdetails
111PEG 4000
211ethylene glycol
311NaCl
411MES
Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropalpha subunit0.2 (M)
101reservoirPEG40003-8 (%)
111reservoirMES40 (mM)
121reservoir0.02 (%)
131reservoirdithiothreitol2 (mM)
141reservoir50 (mM)
21dropbeta subunit1.0-1.5 equivalents each of
31dropssDNA
41drop50 (mM)
51dropTris-HCl5 (mM)
61dropEDTA0.1 (mM)
71drop0.02 (%)
81dropdithiothreitol2 (mM)
91reservoirethylene glycol30 (%)

217705

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